TABLE 4.
Plasmids used in this study
| Plasmid | Relevant genotype and/or descriptiona | Source or reference |
|---|---|---|
| pCR2.1-Topo | Ampr; Topo cloning vector | Invitrogen |
| pCR2.1::pmic-210/0mcpMIADB | Ampr; containing mcpM promoter −210/0 and mcpM, mcpI, mcpA, mcpD, and mcpB | This study |
| pMMB207 | Chlr; RSF1010 derivative; IncQ lacIq Tac oriT | 67 |
| pMMB207::mcpM | Chlr; pMMB207 containing the mcpM gene with a 6×His tag at the C terminus | This study |
| pMMB207::mcpI | Chlr; pMMB207 containing the mcpI gene with a Flag tag at the C terminus | This study |
| pFPV25.1::gfpmut3 | Ampr; vector containing GFPmut3 | 62 |
| pFPV25.1::tdTomato | Ampr; vector containing tdTomato | This study |
| pKD4 | Kanr; containing Kanr cassette for PCR amplification | 68 |
| pKD46 | Ampr | 59 |
| pUT18 | Ampr; cloning vector | Euromedex |
| pUT18C | Ampr; cloning vector | Euromedex |
| pUT18C-Zip | Ampr; pUT18 containing the leucine zipper | Euromedex |
| pUT18::mcpMΔ36 | Ampr; pUT18 containing the mcpMΔ36 gene | This study |
| pUT18::mcpI | Ampr; pUT18 containing the mcpI gene | This study |
| pUT18::ompF | Ampr; pUT18 containing the ompF gene | This study |
| pUT18C::mcpMΔ36 | Ampr; pUT18C containing the mcpMΔ36 gene | This study |
| pUT18C::mcpI | Ampr; pUT18C containing the mcpI gene | This study |
| pUT18C::ompF | Ampr; pUT18C containing the ompF gene | This study |
| pKT25 | Kanr; cloning vector | Euromedex |
| pKT25-Zip | Kanr; pKT25 containing the leucine zipper | This study |
| pKT25::mcpMΔ36 | Kanr; pKT25 containing the mcpMΔ36 gene | This study |
| pKT25::mcpI | Kanr; pKT25 containing the mcpI gene | This study |
| pKT25::ompF | Kanr; pKT25 containing the ompF gene | This study |
| pKNT25 | Kanr; cloning vector | Euromedex |
| pKNT25::mcpMΔ36 | Kanr; pKNT25 containing the mcpMΔ36 gene | This study |
| pKNT25::mcpI | Kanr; pKNT25 containing the mcpI gene | This study |
| pKNT25::ompF | Kanr; pKNT25 containing the ompF gene | This study |
a
Ampr, ampicillin resistant; Chlr, chloramphenicol resistant; Kanr, kanamycin resistant.