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Growth of wild-type A. tumefaciens S33 (black line), S33-ΔetfA-I mutant strain (red line), and S333-ΔetfB-I mutant strain (blue line) in HSP medium (A) and nicotine medium (B). HSP (0.5 g/liter) and nicotine (1 g/liter) were used as the sole sources of carbon and nitrogen in the two media, respectively. Data in figures are the means of the results from triple repeated experiments, and the error bars indicate the standard deviations of the data.
