FIG 6.
Expression levels of etfA-I and etfA-II in wild-type strain A. tumefaciens S33, S33-ΔetfA-I, and S333-ΔetfB-I cultured in LB medium (LB) and nicotine medium (Nic) determined by qRT-PCR analysis. The 16S rRNA gene was used as an internal reference, and the CT values of etfA-I in the wild-type strain cultured in LB medium were used as a baseline. Statistical analysis was performed on 2−ΔΔCT values with normalization to the 16S rRNA gene using paired Student’s t test by SPSS. **, P < 0.001; *, P < 0.05.