FIG 4.

Impact of ramoplanin (A) and bacitracin (B) on entianin-mediated breakdown of the membrane potential using the DiSC₃(5) diffusion assay. B168.KO (without immunity proteins) cells were incubated with the fluorescent dye DiSC₃(5), and fluorescence was monitored (emission, 670 nm; excitation, 544 nm). Asterisks indicate the time of 2.5 μM DiSC₃(5) addition. (A) Ramoplanin applied in equimolar concentration with entianin (130 nM, left) and in 5-fold excess (650 nM, right). +¹, time when entianin (red), valinomycin (blue), or ramoplanin (green) was added; +², time of addition of entianin to cells preincubated with ramoplanin (purple). (B) Bacitracin applied in 1,000-fold molar excess with entianin (130 nM). +¹, time when entianin (red), valinomycin (blue), or bacitracin (green) was added; +², time of further addition of entianin to cells preincubated with bacitracin (purple).