FIG 4.

Inhibition of the yggS mutant by Hse and its alleviation by thrC overexpression. (A to D) Growth curves of the WT_BW^-pU0, ΔyggS_BW^-pU0, and ΔyggS_BW^-YggS strains in liquid M9 medium without additive (A) or with 1 mM Hse (B), 0.1 mM Met and 1 mM Hse (C), or 0.1 mM Ile and 1 mM Hse (D) at 30°C are represented. (E) Growth of strains WT_BW^-pCA24N, ΔyggS_BW^-pCA24N, and ΔyggS_BW^-ThrC in M9 medium containing 1 mM Hse at 30°. (F) Comparison of the growth levels of strains WT_BW^-pBAD24, ΔyggS_BW^-pBAD24, and ΔyggS_BW^-AHASII in M9 medium containing 1 mM Hse at 30°C. Error bars show standard deviations of results from at least three independent experiments.