FIG 5.

Effect of Hse with respect to the metabolites of the Thr, Ile, and Val biosynthetic pathways of the yggS mutant. The intracellular concentrations of amino acids and keto acids of the WT_BW^-pU0 and ΔyggS_BW^-pU0 strains grown in M9 medium supplemented with 1 mM Hse at 30°C are shown in panels A and C, respectively. HPLC chromatograms of the amino acid analyses showing P-Hse accumulation in the ΔyggS_BW^-pU0 strain are shown in panel B. Error bars show standard deviations of results from at least three independent experiments.