TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli WTBW | E. coli K-12 BW25113 wild type; Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) rph-1 Δ(rhaD-rhaB)568 hsdR514 | Keio collection |
| E. coli ΔyggSBW | BW25113 ΔyggS::kan (JW2918-KC) | Keio collection |
| E. coli ΔmetABW | BW25113 ΔmetA::kan (JW3973-KC | Keio collection |
| E. coli ΔthrBBW | BW25113 ΔthrB::kan (JW0002-KC) | Keio collection |
| E. coli ΔthrCBW | BW25113 ΔthrC::kan (JW0003-KC) | Keio collection |
| E. coli JW3973-KCkan- | BW25113 ΔmetA | This study |
| E. coli JW0002-KCkan- | BW25113 ΔthrB | This study |
| E. coli JW0003-KCkan- | BW25113 ΔthrC | This study |
| E. coli ΔmetA ΔyggSBW | BW25113 ΔmetA ΔyggS::kan | This study |
| E. coli ΔthrB ΔyggSBW | BW25113 ΔthrB ΔyggS::kan | This study |
| E. coli ΔthrC ΔyggSBW | BW25113 ΔthrC ΔyggS::kan | This study |
| E. coli WTBW-pu0 | WTBW/pU0 | 1 |
| E. coli WTBW-GFP | WTBW/pBAD18-GFP | This study |
| E. coli WTBW-pBAD24 | WTBW/pBAD24 | This study |
| E. coli WTBW-pBAD | WTBW/pBAD-Myc-HisA | This study |
| E. coli WTBW-pCA24N | WTBW/pCA24N | This study |
| E. coli ΔyggSBW-pu0 | ΔyggSBW/pU0 | 1 |
| E. coli ΔyggSBW-yggS | ΔyggSBW/pUS | 1 |
| E. coli ΔyggSBW-GFP | ΔyggSBW/pBAD-GFP | This study |
| E. coli ΔyggSBW-pBAD24 | ΔyggSBW/pBAD24 | This study |
| E. coli ΔyggSBW-pBAD | ΔyggSBW/pBAD-Myc-HisA | This study |
| E. coli ΔyggSBW-AHASII | ΔyggSBW/pBAD-AHASII | This study |
| E. coli ΔyggSBW-pdxH | ΔyggSBW/pBAD-PdxH | This study |
| E. coli ΔyggSBW-pCA24N | ΔyggSBW/pCA24N | This study |
| E. coli ΔyggSBW-thrC | ΔyggSBW/pCA24N-thrC | This study |
| E. coli ΔmetABW-pu0 | ΔmetABW/pU0 | This study |
| E. coli ΔmetA ΔyggSBW | ΔmetA ΔyggSBW/pU0 | This study |
| E. coli ΔthrBBW-pu0 | ΔthrBBW/pU0 | This study |
| E. coli ΔthrB ΔyggSBW-pu0 | ΔthrB ΔyggSBW/pU0 | This study |
| E. coli ΔthrCBW-pu0 | ΔthrCBW/pU0 | This study |
| E. coli ΔthrC ΔyggSBW-pu0 | ΔthrC ΔyggSBW/pU0 | This study |
| E. coli ΔthrBBW-GFP+ | ΔthrBBW/pBAD-GFP | This study |
| E. coli ΔthrB ΔyggSBW-GFP+ | ΔthrB ΔyggSBW/pBAD-GFP | This study |
| E. coli ΔthrCBW-GFP | ΔthrCBW/pBAD-GFP | This study |
| E. coli ΔthrC ΔyggSBW-GFP | ΔthrC ΔyggSBW/pBAD-GFP | This study |
| Plasmids | ||
| pU0 | pUC19 containing partial sequence of yggS | 1 |
| pUS | pUC19 expressing YggS-His | 1 |
| pCP20 | Expresses yeast Flp recombinase gene | 36 |
| pKD46 | Express lambda red recombinase | 31 |
| pKD13 | Template plasmid | 31 |
| pBAD-GFP | pBAD18/GFP | Laboratory collection |
| pBAD24 | pBAD24 empty vector | Laboratory collection |
| pBAD24-AHASII | pBAD24 containing S. enterica ilvGM (pDM1599) | Laboratory collection |
| pBAD-PdxH | pBAD-Myc-HisC expressing E. coli PdxH | This study |
| pCA24N | pCA24N empty vector | ASKA clone |
| pCA24N-thrC | pCA24N expressing ThrC | ASKA clone |