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. 2019 May 16;85(11):e00066-19. doi: 10.1128/AEM.00066-19

FIG 2.

FIG 2

Construction of the pUC18T-mini-Tn7T-Apr-LAC and pUC18T-mini-Tn7T-Zeo-LAC plasmids. The plasmids were created by replacing the aacC1 gentamicin resistance gene of pUC18T-mini-Tn7T-Gm-LAC with apramycin and zeocin resistance genes, respectively. Plasmid sequences were confirmed using Illumina Hi-Seq next-generation sequencing (MGH CCIB DNA Core Facility, Cambridge, MA, USA). Maps were constructed using SnapGene software (GSL Biotech).