TABLE 2.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristicsa | Source or reference/GenBank accession number |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α(λpir+) | F– φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Kumar lab collection |
| HB101 | F– mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 (Smr) glnV44 λ– | Kumar lab collection |
| SM10(λpir+) | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km λpir+ | Kumar lab collection |
| A. baumannii | ||
| ATCC 17978 | Type strain | ATCC |
| AB030 | XDR clinical isolate from Winnipeg, MB | 27 |
| AB031 | Clinical isolate from Toronto, ON | 27 |
| LAC-4 | MDR clinical isolate from Los Angeles County, CA | 26 |
| AB234 | ATCC 17978::mini-Tn7T-Apr-LAC | This study |
| AB235 | ATCC 17978::mini-Tn7T-Zeo-LAC | This study |
| AB240 | LAC-4::mini-Tn7T-Zeo-LAC | This study |
| AB241 | AB031::mini-Tn7T-Zeo-LAC | This study |
| AB242 | AB030::mini-Tn7T-Apr-LAC | This study |
| AB243 | AB031::mini-Tn7T-Apr-LAC | This study |
| AB244 | LAC-4::mini-Tn7T-Apr-LAC | This study |
| AB271 | ATCC 17978::mini-Tn7T-Apr-mCherry | This study |
| AB272 | LAC-4::mini-Tn7T-Apr-RubyRed | This study |
| AB273 | AB030::mini-Tn7T-Apr-sfGFP | This study |
| AB274 | AB030::mini-Tn7T-Apr-mTurquoise | This study |
| Plasmids | ||
| pUC18T-mini-Tn7T-Gm-LAC | Gmr Ampr | 4 |
| pUC18T-miniTn7T-Gm | Gmr Ampr | 4 |
| pUC18T-miniTn7T-Apr-LAC | Aprr Ampr | This study/MH290812.1 |
| pUC18T-miniTn7T-Apr | Aprr Ampr | This study/MK356268 |
| pUC18T-miniTn7T-Zeo-LAC | Zeor Ampr | This study/MH290813.1 |
| pUC18T-miniTn7T-Zeo | Zeor Ampr | This study/MK356269 |
| pFLP2ab | pFLP2 containing the origin of replication from pWH1266, Ampr | This study |
| pFLP2A | Aprr | This study/MK356266 |
| pFLP2Z | Zeor | This study/MK356267 |
| pFAM1 | Aprr Ampr | Schweizer Lab collection |
| pFZE1 | Zeor Ampr | 5 |
| pTNS2 | Ampr | 4 |
| pRK2013 | Kmr | 28 |
| pUC18T-mini-Tn7T-Apr-sfGFP | Aprr | This study/MH976505 |
| pUC18T-mini-Tn7T-Apr- mCherry | Aprr | This study/MH976504 |
| pUC18T-mini-Tn7T-Apr-mTurquoise | Aprr | This study/MK213339 |
| pUC18T-mini-Tn7T-Apr-RubyRed | Aprr | This study/MK213338 |
| pFPV-mCherry/2 | Ampr | 29 |
| pFPV-mTurquoise2 | Ampr | Chao lab collection. |
| pFPV-mRuby2 | Ampr | Chao lab collection |
| pUC57-sfGFP | Ampr | This study |
a
Abbreviations: Amp, ampicillin; Apr, apramycin; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Zeo, zeocin.