TABLE 2.
Composition of defined artificial medium supplements
| Metabolite | Concn (μg ml−1)a
|
|
|---|---|---|
| Sprouts | Lettuce apoplast | |
| Saccharides (SA) | ||
| Sucrose | 3,021.4 | 2,116.2 |
| Fructose | 1,443.4 | 926.5 |
| Glucose | 1,425.0 | 769.8 |
| Amino acids (AA) | ||
| Asparagine | 814.3 | NA |
| Alanine | 766.1 | NA |
| Serine | 327.4 | NA |
| Oxoproline | NA | 63.4 |
| Organic acids (OA) | ||
| Malic acid | NA | 194.0 |
| 2,3-Dihydroxy-propanoic acid | NA | 143.5 |
a
The concentrations of the major six components in sprout extracts (alfalfa and fenugreek combined) and lettuce apoplast used to generate defined artificial media were determined by HPLC and GC-MS. NA, not applicable.