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. 2019 May 16;85(11):e00123-19. doi: 10.1128/AEM.00123-19

TABLE 3.

Biofilm formation by reference E. coli isolates in plant tissue extractsa

Treatment Avg ± variance density of crystal violet at OD590b
Sakai ZAP1589 JHI5025 JHI5039 MG1655
Sp. apoplast 0.002 ± 0.001 (NS) 0.011 ± 0.001 (NS) 0.372 ± 0.007**** 0.013 ± 0.000 (NS) 0.001 ± 0.002 (NS)
Sp. leaf 0.071 ± 0.000*** 0.128 ± 0.001**** 0.218 ± 0.034**** 0.113 ± 0.001**** 0.000 ± 0.000 (NS)
Sp. root 0.173 ± 0.000**** 0.148 ± 0.017**** 0.179 ± 0.015**** 0.126 ± 0.000**** 0.013 ± 0.000 (NS)
Lt. apoplast 0.000 ± 0.002 (NS) 0.005 ± 0.000 (NS) 0.125 ± 0.005**** 0.001 ± 0.000 (NS) 0.000 ± 0.000 (NS)
Lt. leaf 0.000 ± 0.000 (NS) 0.018 ± 0.001 (NS) 0.151 ± 0.002**** 0.007 ± 0.000 (NS) 0.001 ± 0.000 (NS)
Lt. root 0.008 ± 0.000 (NS) 0.029 ± 0.001 (NS) 0.066 ± 0.001 (NS) 0.025 ± 0.000 (NS) 0.000 ± 0.000 (NS)
RDMG 0.000 ± 0.000 0.000 ± 0.000 0.013 ± 0.000 0.000 ± 0.000 0.000 ± 0.000
a

Biofilms were formed on polystyrene multiwall plates following incubation in spinach (Sp.) and lettuce (Lt.) extracts (apoplast, leaf, root) and rich defined MOPS medium with glycerol (RDMG) at 18°C for 48 h under static conditions.

b

P value summaries are provided per isolate for each extract type versus RDMG (NS, not significant [P > 0.05]; ***, P ≤ 0.001; ****, P ≤ 0.0001).