TABLE 2.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Description or sequence | Reference or source |
|---|---|---|
| Strains | ||
| Paraburkholderia phytofirmans | ||
| PsJN | Wild type | Lab stock |
| PsJN::GFP | PsJN tagged with GFP; Kmr | Lab stock |
| PsJN ΔhscA | Mutant with deletion of hscA gene | This study |
| PsJN ΔiscS | Mutant with deletion of iscS gene | This study |
| PsJN::dsRed | PsJN tagged with dsRed; Cmr | This study |
| Saccharomyces cerevisiae InvSc1 | Yeast strain for in vivo recombination; MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1 | Invitrogen (Carlsbad, CA, USA) |
| Escherichia coli | ||
| WM3064 | Strain for conjugation; λpir, DAP auxotroph | 85 |
| DH5α | Host for cloning | Lab stock |
| Plasmids | ||
| pin29 | Nonmobilizable plasmid pIN29; oripBBR Δmob Cmr dsRed | 83 |
| pMQ30 | 7.6-kb mobilizable suicide vector used for gene replacement in Pseudomonas: sacB URA3 Gmr | 75 |
| pMQ30-ΔhscA | pMQ30 containing two fragments of ISC genes Bphyt_2574 and Bphyt_2576 | This study |
| pMQ30-ΔiscS | pMQ30 containing two fragments of ISC genes Bphyt_2578 and Bphyt_2580 | This study |
| Primers (5′ → 3′) | ||
| UphscAF | GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTGGAACGACAGCCACTTCGAC | This study |
| UphscAR | GCTTCACCACCACAGATGCCTTGCCACGACCCATGAAG | This study |
| DhscAF | CTTCATGGGTCGTGGCAAGGCATCTGTGGTGGTGAAGC | This study |
| DhscAR | CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTCGACAACCAGATCCTGCT | This study |
| UpiscSF | GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTGCGATGATTGACCTGGCACTG | This study |
| UpiscSR | TTTCCGTCGCCATTTCTTCGACCCGAGGTCCAGATGATTT | This study |
| DiscSF | AAATCATCTGGACCTCGGGTCGAAGAAATGGCGACGGAAA | This study |
| DiscSR | CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATCCGTGACGTTGCTTGTAGTC | This study |
| Bphyt_0126F | CGTCATCCCCTTCGGTATGG | This study |
| Bphyt_0126R | ATACGGGAACAGGGACATGC | This study |
| Bphyt_0127F | GATCGTATGCCTGACCTGGG | This study |
| Bphyt_0127R | CCTGCATTGCCTTTCCGATG | This study |
| Bphyt_4275F | ATGCTGAAGGACACCTTCCC | This study |
| Bphyt_4275R | AAAGCCGAACGACTGTTCCT | This study |
| Bphyt_4277F | TGTTATTGCGCGACCTGGTA | This study |
| Bphyt_4277R | GGTCCATTTCAGGCATTCGC | This study |
| Bphyt_6042R | TTGCCTACTTTCTCGGGCTG | This study |
| Bphyt_6042F | CACATAGGCCTGAGCGACAT | This study |
| 16S-F | AAGCCTGATCCAGCAATGCC | This study |
| 16S-R | TCCGATTAACGCTTGCACCC | This study |