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. Author manuscript; available in PMC: 2019 Aug 21.
Published in final edited form as: Cell. 2019 Jan 31;176(5):982–997.e16. doi: 10.1016/j.cell.2018.12.031

Figure 3. Skin ILCs Are Tissue-Resident during Steady State and Inflammation.

Figure 3.

(A) Percentage of GFP+ cells from H2B-GFP;R26-M2rtTA mice that were treated with doxycycline for 6 days to induce GFP expression. GFP+ cells were detected by flow cytometry at indicated time points.

(B) Percentage of BrdU+ cells from WT mice that were treated with BrdU for 7 days. BrdU+ cells were detected by flow cytometry on day 8.

(C) Percentage of CD45.1+ (donor) and CD45.2+ (host) cells from bone marrow chimeric mice. CD45.1+ bone marrow cells were transferred into lethally irradiated CD45.2+ mice. Chimerism was analyzed by flow cytometry 8 weeks after bone marrow transplantation.

(D and E) Percentage of host-derived cells from skin and peripheral blood of parabiotic mice, wherein CD45.1+ and CD45.2+ congenic mice were surgically connected. Chimerism was analyzed by flow cytometry 8 weeks after surgery. (E) Both parabiotic partners were treated with MC903 every other day for 12 days.

(A to E) The data are shown as mean ± SD and were pooled from more than two independent experiments (n = 3 to 4 per experiment). Each dot represents an individual mouse. ANOVA with Tukey’s multiple comparison test was used to measure significance. *p < 0.05, **p < 0.01 and ***p < 0.001.