Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 16;10:1105. doi: 10.3389/fimmu.2019.01105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Kirpach, Colone, Bürckert, Faison, Dubois, Sinner, Reye and Muller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of HTS sequence clustering. (A) Mixed clones obtained using Change-O (67) that contained sequences of both IgM and IgG isotypes were selected and the percentage of the total repertoire (all samples from the same donor pooled) they represent is shown. (B) Correlation between average IgM and IgG CDR3aa sequence overlap between timepoints for the different patients (closed symbols) and controls (open symbols). (C) Correlation between IgG CDR3aa sequence overlap between timepoints (average) and clustering between donors (median) (44, 48, 68) is represented. Spearman's Rank Correlation Hypothesis Testing was done manually in Excel. (D) Sequence clustering between all donors was determined using the python script from Galson et al. (44, 48, 68). Median values were calculated as before (Figure 4) but here the sequence overlap between samples belonging to different groups was also assessed. Lines connect values of the same donor. The boxes show the number of sequence overlap values calculated and used to generate the median values presented. The brackets give the number of donors for which this number of sequence overlap values was used to calculate the median value shown. Dashed line: 5/12 (42%) acute patients but only 1/11 (9%) controls showed median values above this threshold. (E,F) Distribution of overlapping clusters among donors.