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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2018 Jul 25;1864(10):3353–3367. doi: 10.1016/j.bbadis.2018.07.022

Figure 1.

Figure 1.

Tafazzin protein and total CL levels are reduced in TAZ kd mice.

A: Dox-inducible knockdown of TAZ protein was determined by immunoblot using heart isolated mitochondrial (12.5 μg) or total brain homogenates (30 μg). Citrate synthase (Cit) was used as the protein loading control. (B) Protein levels of TAZ were quantitated by densitometry and normalized against citrate synthase (n=1–3). Liquid chromatography-mass spectrometry was used to quantitate phospholipid amounts in whole brain (n=8). Total phospholipid values are expressed as a % change in the TAZ kd mice (Tg +dox) compared to the littermate (NTg +dox) controls [((NTg+dox) – (Tg+dox))/(NTg +dox)x100](C) or pmol/nmol total PL (D). Phosphatidylglycerol (E) and cardiolipin (F) content was measured in liver and heart homogenates by phosphorus assay (n=3). (G) mRNA levels of the indicated genes were measured by qPCR and normalized to TFIIB (n=11–13). Data are means ±SD. *p<0.05 compared with NTg +dox mice. †p<0.05 Tg +dox compared to all other groups.