FIG 8.

Antibody and CD4 binding properties of cells expressing a second-generation engineered Env from the DU422 isolate. Soluble CD4 and bNAb binding to cells transiently expressing full-length Env from the DU422 isolate was measured by flow cytometry. Wild-type Env is gray, and the engineered mutant (DU422-QES.c12 carrying seven mutations) is blue. (A) PG16; (B) sCD4 (D1-D2 domains); (C) PGT145; (D) PGT121; (E) PGT128; (F) 35O22. The data are means (n = 2), with error bars showing the range. Comparable binding data for engineered Envs from 15 different strains are summarized in Table 2.