FIG 3.

Virus reactivation in ex vivo memory CD4⁺ T cells and resting CD4⁺ T cells generated in vitro. (A) Purified ex vivo CD4⁺ T cells and expanded CD4⁺ T cells with more than 35 days in culture were treated with prostratin (1 μM) or DMSO (control) for 24 h, and the culture supernatants were collected to evaluate virus production. (B) Purified ex vivo CD4⁺ T cells and expanded CD4⁺ T cells in two independent expansions (experiments 1 and 2) from two representative donors were treated with different classes of LRAs for 24 h, and the culture supernatants were collected to evaluate virus production. The HIV copy numbers are shown with the standard deviations. (C) Cryopreserved expanded CD4⁺ T cells were recovered and treated for 3 days under 15 conditions, including 10 LRAs and their combinations. Culture supernatants were collected to evaluate virus production.