FIG 6.

The I170P Env incompatibility is partially alleviated by replacing MLV MA. (A) Schematic diagram of chimeric MLV Gag constructs used. (B) Western blot of purified HIV-1 and MLV particles with the indicated Env shows Env incorporation and capsid release. Averages and standard deviations from six independent experiments including infectivity from medium used for Western blotting are shown. The Western blot image is a representative example of two independent experiments. (C) Relative percent infectivity normalized to that of HIV-1 with MLV/GaLV Δ16. Averages and standard deviations from three independent experiments are shown. (D) Virus capture assay with MLV, hMA-MLV, and Src-MLV Gag with iGluc vectors and GFP-tagged Envs. Env incorporation was represented by signal from the infected cells with captured virus. Each sample was normalized to the level of positive control (straight infection without wash). Averages and standard deviations from three independent experiments are shown.