FIG 11.
M2L deletion does not affect the oncolytic activities of the vaccinia virus in vivo. A total of 5 × 106 HCT116 human tumoral cells was implanted subcutaneously in nude mice. When the tumor size reached 100 to 200 mm3 (∼10 days after cell implantation), mice were treated with one intravenous administration of 105 or 107 PFU of doubly (TK− RR−) or triply (TK− RR−M2L−) deleted vaccinia virus encoding firefly luciferase (VV-DD-Luc [red curves] or VV-TD-Luc [green curves], respectively). Tumor volume was measured twice weekly. Each represented value is the mean of the tumor volume (± standard deviation) of 9 animals.