FIG 2.

Culture supernatants of orthopoxvirus-infected HeLa cells inhibit the binding of hCTLA4 to hCD80. HeLa cells were infected at an MOI of 1 by different poxviruses, and the culture supernatants were recovered 48 h after infection. The supernatants were centrifuged, filtered on a 0.2-μm-pore-size filter to remove most of the virus, and tested for their capacity to inhibit the CD80-CTLA4 interaction as described in the legend of Fig. 1. Each represented value is the mean (± standard deviation) of triplicate wells. MVA, modified vaccinia virus Ankara; Cop VV, Copenhagen vaccinia virus; WR VV, vaccinia virus Western reserve; Wyeth VV, vaccinia virus Wyeth; RCNV, raccoonpox virus; RPXV, rabbitpox virus; CPXV, cowpox virus; BPSV, bovine papular stomatitis virus; CTV, Cotia virus; FPV, fowlpox virus; MYXV, myxoma virus; ORFV, ORF virus; PCPV, pseudocowpox virus; SQFV, squirrel fibroma virus; SWPV, Swinepox virus; YLDV, Yaba-like disease virus.