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Functional activity of SC3 founder (V08-1), early (V10-2), and late (V20-1) RREs. The functional activity of the three RREs was determined by transfecting reporter constructs containing the different RREs into 293T/17 cells along with 50 ng of SC3 Rev and measuring the ratio of the mean fluorescent intensity of GFP to that of BFP. The SEM is represented by error bars (n = 4). *, P < 0.05; NS, nonsignificant difference.
