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Soluble protein purification. His-tagged soluble forms of HSV-1 glycoprotein D (gD1) and B (gB1) were affinity purified. Soluble HCV envelope protein E2 (HCV E2) was purified as a control. Protein stocks were validated using human monoclonal antibodies specific for the glycoproteins and a primary antibody specific for His-tagged proteins. Means ± standard deviations (SD) are reported.
