FIG 4.

Evaluation of serum activity against HSV-1 and -2 infections. (A) Neutralizing (NT) activity of 44 sera was assessed on Vero E6 cells, and results show that HSV-1 (▲) was more efficiently blocked than HSV-2 (●). (B) When tested in PEI assay on the same cells, no samples (except one) impaired virus replication efficiently, although higher activity again was observed against HSV-1 (▲) than HSV-2 (●). Sera were used at 1:200 dilution. Median values with interquartile ranges are reported for each experiment (****, P < 0.0001). Each data point is represented by its stratification color: sera able to bind only HSV-1 (light red) or only HSV-2 (light green), sera that bind both viruses equally (gray), sera that preferentially HSV-1 over HSV-2 (red) or vice versa (green), and negative sera (black).