FIG 5.

Evaluation of purified IgG activity against HSV-1 and -2 infections. PEI assay was performed on Vero E6 cells using lower dilutions of the neutralizing (NT) serum (1:100) control and purified total IgGs obtained from the same serum sample (100 μg/ml). Nonneutralizing (non-NT) serum and its purified total IgG fraction were used as a negative control. Mean values ± SD are reported for each experimental condition (***, P < 0.001; ****, P < 0.0001). Titration results for anti-gD and anti-gB present in NT and non-NT sera and their corresponding purified IgG fractions are indicated under the graph. Median values are reported.