TABLE 1.
Mutant UL138 alleles used in this study
| Mutant name | Mutant explanation | Specific substitutions |
Corresponding mutant virus |
Reference(s) or source |
|---|---|---|---|---|
| WT | No mutations in UL138 | TB138-HA or TB138-Flag | 29, 64 | |
| mY-1 | Mutation of first tyrosine motif | Y26LAY to A26AAY | This study | |
| mY-2 | Mutation of second tyrosine motif | Y44RWL to A44AAA | This study | |
| mY-3 | Mutation of third tyrosine motif | Y54GEY to A54GEY | This study | |
| mLL | Mutation of acidic cluster dileucine motif | D142VDLL to A142AAAA | TB138 mLL-HA | This study |
| Δ4 | Mutation of four Golgi motifs | The mutations listed above combined in one allele | TB138 Δ4-HA | This study |
| M1A | Transmembrane mutant; expresses only the short isoform of UL138 from M16 | M1 to A1 | TB138 M1A-Flag | 29 |
| M16A | Expresses only the full-length long isoform of UL138 from M1 | M16 to A16 | TB138 M16A-Flag | 29 |
| M1AΔ4 | Mutation of four Golgi motifs in the context of the transmembrane mutant; M1A | M1 to A1 combined with the Δ4 mutations | This study |