TABLE 2.
Primers used in this study
| Use | Primer namea | Primer sequence |
|---|---|---|
| UL138 SDM primers | UL138 mY-1 SDM Forward | 5′-GATCGTGGCCATTCTCTGCGCGGCCGCTTACCATTGGCACGACAC |
| UL138 mY-1 SDM Reverse | 5′-GTGTCGTGCCAATGGTAAGCGGCCGCGCAGAGAATGGCCACGATC | |
| UL138 mY-2 SDM Forward | 5′-GTGCGCATGTTTTTGAGCGCCGCGGCGGCGATCCGCTGTTGCGAGCTGTAC | |
| UL138 mY-2 SDM Reverse | 5′-GTACAGCTCGCAACAGCGGATCGCCGCCGCGGCGCTCAAAAACATGCGCAC | |
| UL138 mY-3 SDM Forward | 5′-CGCTGTTGCGAGCTGGCCGGCGAATACGAGCGCCGGTTC | |
| UL138 mY-3 SDM Reverse | 5′-GAACCGGCGCTCGTATTCGCCGGCCAGCTCGCAACAGCG | |
| UL138 mLL SDM Forward | 5′-GGTGACGGCGCCGCTGACCGCGGCGGCGGCGGCGAAACCCGTGACGGGATCC | |
| UL138 mLL SDM Reverse | 5′-GGATCCCGTCACGGGTTTCGCCGCCGCCGCCGCGGTCAGCGGCGCCGTCACC | |
| UL138-eGFP fusion cloning | UL138 eGFP fusion Forward | 5′-TATGACGTGCCTGACTATGCCGTGAGCAAGGGCGAGGAGCTGTTC |
| UL138 eGFP fusion Reverse | 5′-AGCTCCTCGCCCTTGCTCACGGCATAGTCAGGCACGTCATAAG | |
| UL138 pSG5 infusion Forward | 5′-ACTATAGGGCGAATTCGATTCCGAAATTCATGGACGATC | |
| eGFP pSG5 infusion Reverse | 5′-ATCTGGATCCGAATTCTTACTTGTACAGCTCGTCCATGCCGAG | |
| HaloTag cloning | Halo pSG5 infusion Forward | 5′-ACTATAGGGCGAATTCATGGCAGAAATCGGTACTGGCTTTC |
| Halo pSG5 infusion Reverse | 5′-ATCTGGATCCGAATTCTCAGGCATAGTCAGGCACGTCATAAGGATAGCCGGAAATCTCGAGCGTCGACAG | |
| BAC mutagenesis primers | UL138 ins KanR-BglII F | 5′-TTGAGTAGATCTTGGTCCGTTGGGAGGAAGTGTCTTCCCAGTGCAGCTACGCGTCGTCGCGGAACCCACTGCTTACTGGCTTATCG |
| UL138 ins KanR-BglII R | 5′-TTTCGTAGATCTGCCAGTGTTACAACCAATTAACC | |
| TB40E-BAC ins UL138 F | 5′-TGTACAAAAGAGAGAGACTGGGACGTAGATCCGGACAGAGGACGGTCACCATGGACGATCTGCCGCTGAAC | |
| TB40E-BAC ins UL138 R | 5′-GTCAAAACGACATTACCGCGATCCGCTCCCCTCTTTTTTCTTTTTCTCATTCAGGCATAGTCAGGCACGTC | |
a
F, forward; R, reverse.