FIG 3.
Binding ability of fusion inhibitors with the target cell membrane. The lipopeptide inhibitors LP-52, LP-80, LP-83, and LP-86 and the control inhibitors T-20, C34, and P-52 were preincubated with TZM-b1 cells, followed by thorough washes, and their sustained activities in inhibiting the infectious molecular clones HIV-1NL4-3 (A) and HIV-1JRCSF (B) were measured. LP-52, LP-80, LP-83, and LP-86 were used at 0.5 nM, while T-20, C34, and P-52 were used at 750, 25, and 200 nM, respectively. (C to F) The IC50 values of LP-83 and LP-86, administered with or without the washing steps, on HIV-1NL4-3 and HIV-1JRCSF were determined. The experiments were performed 3 times, and data are expressed as means ± standard deviations (SDs).