TABLE 2.
List of primer sets used for PCR confirmation of RHDV2
| Primer name | Sequence (5′→3′) | Amplicon size | Gene |
|---|---|---|---|
| Derived from assembled contigs | |||
| BR_RHDV1_fwd | CTTGGCCAGTCTTTCTGGAG | 168 | RdRp |
| BR_RHDV1_Rev | ACAGGCAAACAGGTCCAAAC | ||
| BR_RHDV3_fwd | ATCCCGCGCCGTACTGGGTTCCATTAG | 405 | RdRp |
| BR_RHDV3_Rev | GGGTCGGGTTTGGTGGGATCTGGAACA | ||
| RHDV strain-specific multiplex (59) | |||
| GI.1a-Aus_fwd | GCGTGGCATTGTGCGCAGCATC | 562 | RdRp |
| GI.1a-Aus_rev | TGTTGGTGATAAGCCATAATCGCG | ||
| GI.1c_fwd | AGCAAGACTGTTGACTCAATTTCG | 435 | VP60 |
| GI.1c_rev | AGGCCTGCACAGTCGTAACGTT | ||
| GI.2_fwd | TTTCCCTGGAAGCAGTTCGTCA | 336 | VP60 |
| GI.2_rev | TGTTGTCTGGTTTATGCCATTTGC | ||
| GI.1a-K5_fwd | TTTATAGATGTATGCCCGCTCAAC | 263 | RdRp |
| GI.1a-K5_rev | CCGTTCGAGTTCCTTGCGGACG |