FIG 1.

Broadly neutralizing antibodies but not nonneutralizing antibodies induce Env internalization. A panel of bNAbs (PGT121, PGT126, PG9, and 2G12) and nNAbs (A32, N5-i5, 17b, and N12-i2) were used to stain the surface of primary CD4⁺ T cells infected with the NL4.3 GFP ADA virus. (A) Histograms depicting representative staining of infected cells (GFP⁺) with A32, 17b, PGT126, and 2G12 MAbs over time or with mock-infected cells (gray). (B and C) Quantification of remaining antibody-Env complexes on the cell-surface over different time points is expressed as percentage of the MFI relative to the 0-min time control. Error bars indicate means ± the standard errors of the mean (SEM). Statistical significance was tested using an unpaired t test or a Mann-Whitney U test based on statistical normality (****, P < 0.0001).