Fig. 5.

The inhibitory effects of EE02 on EGFR/Eps8 complex-associated signaling. a The results of protein chip assays are shown. MDA-MB-468 cells were incubated with DMSO or EE02 (10 μM) for 24 h, and then total protein was extracted for protein chip assays. b Western blot assays for the expression of EGFR, EPS8, Akt, p-Akt, Erk and p-Erk were performed with MDA-MB-468 cells treated with increasing concentrations of EE02 for 24 h. c Western blot assays for the expression of EGFR, EPS8, Akt, p-Akt, Erk and p-Erk were performed with H460 cells treated with increasing concentrations of EE02 for 24 h