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. 2019 May 22;19:484. doi: 10.1186/s12885-019-5712-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Reduction in the myeloid immune compartment in the absence of NK cells PEDF does not reduce hepatic metastases. a-c Excised livers from all groups were macerated for RNA extraction, cDNA synthesis and examination of mRNA expression. a Gene expression analyses of the type-1 genes Socs3 and Tnfa mRNA and the type-2 Arg1 and Tgfb mRNA using the ∆C_T Comparative method. b The pro-apoptotic Casp3 mRNA and the tumor suppressor Trp53 were examined using the ∆C_T Comparative method, as well. c Cd68 mRNA, typical of infiltrative macrophages, was examined in the bulk liver tissue. All Data represents mean ± SEM. All mRNA analyses display the fold change of target gene over endogenous control (Gapdh)