Table 2.

Summary of RNA sequencing metrics

Sample	Yield (Mbp)	N Reads	%Q30	Mean Q	N Reads after trimming	% Mapping
CAF1	8.297	33,187,088	94.98	35.76	30,158,494	71.4
CAF2	7.166	28,665,463	94.84	35.7	26,025,539	70.9
CAF3	6.782	27,126,385	94.96	35.75	24,736,334	71.5
COF1	9.01	36,040,513	95.38	35.8	32,867,578	69.7
COF2	11.272	45,089,307	95.29	35.78	41,064,935	70.2
COF3	6.922	27,686,685	95.38	35.81	25,285,497	73

Yield (Mbp) number of bases in mega bases, Q quality score, %Q30 percentage of bases with a quality score of at least 30. CAF cold acclimated females, COF Control Females