Table 4.
Sequence analysis of the BCV spike gene. Sequence analysis of a 1102 nucleotide fragment of the BCV spike gene spanning the S1 hypervariable region and antigenic domain II
| Position in Mebus spike peptide (U00735.2)a | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 458 | 465 | 470 | 476 | 484 | 492 | 509 | 510 | 525 | 531 | 535 | 543 | 546 | 571 | 578 | 628 | |
| Mebus | F | V | H | A | S | D | N | S | H | N | T | S | P | Y | T | L |
| 1701_PC_1b | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2018_PC_1 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2507_PC_2 | S | A | D | V | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2369_PC_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2785_PC_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2830_PC_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 1399_PC_3 | F c | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | F |
| 4150_PC_3 | S | A | D | A | T | G | T | T | Y | D | I | A | S | H | S | L |
| 2402_MT_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2507_MT_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2812_MT_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2815_MT_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2826_MT_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2830_MT_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
| 2721_W_2 | S | A | D | A | T | G | T | T | Y | D | T | A | S | H | S | L |
aInferred polypeptide sequences were aligned with the reference Mebus genome (Genbank accession U00735.2) corresponding to amino acids 396 to 762, and only those amino acid positions differing from the reference Mebus strain are pictured
bStrains from study calves are listed as: animal ID_sample acquisition time_herd number. PC = preconditioning, MT = mass treatment, W = weaning
cBold letters are the four amino acid changes inferred by the single nucleotide polymorphisms identified by Sanger sequencing