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Development of a transient assay to test sgRNA efficiency for CRISPR editing in vivo. Model showing assay components and their predicted interactions. The symbols for the corresponding genomic sequence, transcript sequence, and protein product of the same gene are drawn in the same color. Gray designates deactivated modules in the reporter construct before or after CRISPR editing events. GOI: gene of interest; fs: frame-shifted; 2A: 2A peptide
