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. 2019 May 23;12:130. doi: 10.1186/s13068-019-1467-y

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Zygosity of T1-edited GONST2 plants. Following isolation of leaf DNA, genomic sequences spanning GONST2_gRNA1 or GONST2_gRNA2 target site were PCR amplified and analyzed by Sanger sequencing. Transgenic plants of each GONST2_gRNA line were classified based on mutation rate at the projected target site