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. 2019 May 16;10:613. doi: 10.3389/fpls.2019.00613

FIGURE 2.

FIGURE 2

FISH mapping of 35S/5S rDNA, TcSAT1, TcSAT2 and TcSAT3 satellite DNA repeats and Arabidopsis-type (TTTAGGG)n telomeric repeats on somatic metaphase chromosomes of Tanacetum cinerariifolium (2n = 18). (a–i) FISH on metaphase chromosomes that evolved linked arrangement of rRNA genes. (a–c) accession 20-7 and (g–i) accession 2158-4 have five linked 5S/35S rDNA sites. (d–f) accession 53-3 has six linked 5S/35S rDNA sites. Arrows on (b,h) mark heteromorphic chromosome pairs 9 and 7 and 8, respectively. The 35S and 5S rDNA loci are labeled in green and red, respectively. (j–l) FISH mapping of TcSAT1 (green) and TcSAT2 repeats (red). Overlapped TcSAT1 and TcSAT2 signals are marked with arrows. (m) FISH mapping of TcSAT3 repeat family (red) and 5S rDNA (green). (n–q) FISH mapping of the TcSAT1 (red) and Arabidopsis-type plant telomeric DNA probe (green). (j–l), (n–q) accession 53-3. (m) accession 2158-5. Scale bar, 10 μm.