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. 2019 May 16;10:613. doi: 10.3389/fpls.2019.00613

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Copyright © 2019 Mlinarec, Skuhala, Jurković, Malenica, McCann, Weiss-Schneeweiss, Bohanec and Besendorfer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FISH mapping of TcSAT1 and TcSAT2 satellite DNA repeats and Arabidopsis-type (TTTAGGG)_n telomeric repeats on meiotic pachytene (a–d) and diakinesis (e–g) chromosomes of T. cinerariifolium (2n = 18). (a) Rabl formation showed with telomeric sequence TTTAGGG (red) and TcSAT1 (green) as probes. (b–g) TcSAT1 (green) and/or TcSAT2 (red). (a,g) accession Africa-7, (c,d) accession 32-2, (e,f) accession 53-3. Arrow in (b–d) mark association of TcSAT1 and TcSAT2 sites. Bivalents in (f) are arranged according to metaphase chromosomes shown on the Figure 2p. Arrow in (f) points to a bivalents that show heterozygosity of subtelomeric heterochromatin. Arrow in (g) indicates connection between non-homologous chromosomes. Scale bar, 10 μm.