FIGURE 1.

LdCen^−/− immunization induce the expression of Th17 cells promoting cytokines and inhibit Th2 cytokines in splenic DCs isolated from infected C57BL/6 mice. (A) Infected cells were sorted from the spleen of different groups of mice after indicated period of postinfection with either LdWT-RFP or LdCen^−/−-mCherry by gating live single cells for (lineage [T cells, B cells, NK cells, and macrophages]⁻ and RFP/mCherry⁺ Cd11c⁺) cells. (B–G) mRNA expression levels of IL-1β, IL-6, TGF-β1, IL-23, IL-27, and IL-10 were measured from infected DCs, and expressed as fold increase over naive DCs. The data represent the mean values + SEM of results from two independent experiments; in each experiment more than eight mice splenocytes were pooled to produce enough infected DCs. *p < 0.05, **p < 0.005.