FIGURE 7.

LdCen^−/−-immunized IL-23R^−/− mice failed to induce host protection upon virulent challenge with L. donovani parasites. Normal mice and IL-23R^−/− mice were immunized with LdCen^−/− and at 5 wk postimmunization mice were sacrificed. Cell specific cytokine secretions were measured by flow cytometry. (A) Gating strategy and representative flow plots; (B and C) bar diagrams represent IL-17–producing CD4 and CD8 T cells; and (D and E) IFN-γ-producing CD4 and CD8 T cells. (F) Spleen parasite burden was measured by serial dilution after 10 wk postchallenge with LdWT (MHOM/SD/62/1S) parasites. The data represent the mean values + SEM of results from three independent experiments. *p < 0.05, **p < 0.005.