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. Author manuscript; available in PMC: 2019 Sep 22.
Published in final edited form as: Nat Chem. 2019 Mar 22;11(4):342–350. doi: 10.1038/s41557-019-0230-0

Figure 5. Immunosuppressive activity of gracilin A derivatives.

Figure 5

Kinetic equilibrium dissociation constants (KD, μM) for binding of gracilin A derivatives to CypA as measured by SPR (n=4, Mean +/− SEM), interleukin–2 release inhibition (IL-2 (IC50, n=10, mean, CI)) by ELISA, and cell viability of human T lymphocytes (Viability (EC50, n=3, mean, CI)) as determined by an MTT assay. CI (95% confidence interval); R2 values (0.91–0.98) were in acceptable range (see Supplementary, Sect. B for details). Inhibition of calcineurin (CN) phosphatase activity at 1 µM expressed in percentage of control cells (n=4, mean +/− SEM). (>10 = not active up to 10 µM).