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. 2019 May 16;10:649. doi: 10.3389/fpls.2019.00649

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Copyright © 2019 Tanner, Colgrave, Blundell, Howitt and Bacic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of maturity (days post-anthesis, DPA) on the accumulation of representative hordein family-specific peptides following trypsin cleavage. The mean multiple reaction monitoring (MRM) peak area ± SE (n = 3) from 5 μg extracted protein is shown for peptides mapping to: (A) avenin-like proteins (ALPs) F2EGD5-T1 (, left y-axis) and M0VEH1-T1 (, right y-axis); (B) B1-hordeins I6TRT2-T1 (),I6TRT2-2 (), I6TRT2-3 (), and; (C) B3-hordeins Q4G3S1-1 (, left y-axis), Q4G3S1-2 (, left y-axis), and I6SJ26-T1 (, right y-axis); (D) D-hordein I6TRS8-T1 (, left y-axis), I6TRS8-T4 (, right y-axis), and I6TRS8-T5 (, right y-axis); (E) γ1-hordein I6TMV6-T1 (, left-y axis), TC131355-T1 (, right y-axis), I6TMV6-T5 (, left y-axis); and (F) γ3-hordein I6TEV2-T1 (), I6TEV2-T2 (). The sequence of proteins and peptide markers are presented in Supplementary Table S4. For clarity, symbols of significance are not shown, but where points differ by 2 × SE, they are significantly different. Detailed statistical comparisons are shown in Supplementary Figures.

Inline graphic — The effect of maturity (days post-anthesis, DPA) on the accumulation of representative hordein family-specific peptides following trypsin cleavage. The mean multiple reaction monitoring (MRM) peak area ± SE (n = 3) from 5 μg extracted protein is shown for peptides mapping to: (A) avenin-like proteins (ALPs) F2EGD5-T1 (, left y-axis) and M0VEH1-T1 (, right y-axis); (B) B1-hordeins I6TRT2-T1 (),I6TRT2-2 (), I6TRT2-3 (), and; (C) B3-hordeins Q4G3S1-1 (, left y-axis), Q4G3S1-2 (, left y-axis), and I6SJ26-T1 (, right y-axis); (D) D-hordein I6TRS8-T1 (, left y-axis), I6TRS8-T4 (, right y-axis), and I6TRS8-T5 (, right y-axis); (E) γ1-hordein I6TMV6-T1 (, left-y axis), TC131355-T1 (, right y-axis), I6TMV6-T5 (, left y-axis); and (F) γ3-hordein I6TEV2-T1 (), I6TEV2-T2 (). The sequence of proteins and peptide markers are presented in Supplementary Table S4. For clarity, symbols of significance are not shown, but where points differ by 2 × SE, they are significantly different. Detailed statistical comparisons are shown in Supplementary Figures.