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. 2019 May 16;10:649. doi: 10.3389/fpls.2019.00649

FIGURE 4.

FIGURE 4

The effect of maturity (days post-anthesis, DPA) on the accumulation of representative hordein family-specific peptides following trypsin cleavage. The mean multiple reaction monitoring (MRM) peak area ± SE (n = 3) from 5 μg extracted protein is shown for peptides mapping to: (A) avenin-like proteins (ALPs) F2EGD5-T1 (Inline graphic, left y-axis) and M0VEH1-T1 (Inline graphic, right y-axis); (B) B1-hordeins I6TRT2-T1 (Inline graphic),I6TRT2-2 (Inline graphic), I6TRT2-3 (Inline graphic), and; (C) B3-hordeins Q4G3S1-1 (Inline graphic, left y-axis), Q4G3S1-2 (Inline graphic, left y-axis), and I6SJ26-T1 (Inline graphic, right y-axis); (D) D-hordein I6TRS8-T1 (Inline graphic, left y-axis), I6TRS8-T4 (Inline graphic, right y-axis), and I6TRS8-T5 (Inline graphic, right y-axis); (E) γ1-hordein I6TMV6-T1 (Inline graphic, left-y axis), TC131355-T1 (Inline graphic, right y-axis), I6TMV6-T5 (Inline graphic, left y-axis); and (F) γ3-hordein I6TEV2-T1 (Inline graphic), I6TEV2-T2 (Inline graphic). The sequence of proteins and peptide markers are presented in Supplementary Table S4. For clarity, symbols of significance are not shown, but where points differ by 2 × SE, they are significantly different. Detailed statistical comparisons are shown in Supplementary Figures.