Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 16;10:649. doi: 10.3389/fpls.2019.00649

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Tanner, Colgrave, Blundell, Howitt and Bacic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The effect of maturity (days post-anthesis, DPA) on the accumulation of representative hordein family-specific peptides following chymotrypsin cleavage. The mean multiple reaction monitoring (MRM) peak area ± SE (n = 3) from 5 μg extracted protein is shown for peptides mapping to: (A) B1-hordeins P06470-C1 (, right y-axis), P06470-C2 (, left y-axis), and P06470-C3 (, right y-axis); (B): B3-hordeins I6SW30-C1 (, left y-axis), I6SJ26-C1 , right y-axis), I6SJ26-C2 (, left y-axis); (C) C-hordeins Q40053-C1 (), Q40053-C3 () and Q41210-C4 (); (D) D-hordein I6TRS8-C1 (), I6TRS8-C5 () and I6TRS8-C6 (); (E) γ1-hordein TC131355-C1 (), γ1-hordein I6TMV6-C1 (); (F) γ3-hordein I6TEV2-C1 (, left y-axis), γ3 I6TEV2-C2 (, right y-axis), and γ3 I6TEV2-C3 (, right y-axis). The sequence of the proteins and peptide markers are shown in Supplementary Table S4. Where the SE is less than symbol size it is not shown. For clarity, symbols of significance are not shown but where points differ by 2 × SE they are significantly different. Detailed statistical comparisons are shown in Supplementary Figures.

Inline graphic — The effect of maturity (days post-anthesis, DPA) on the accumulation of representative hordein family-specific peptides following chymotrypsin cleavage. The mean multiple reaction monitoring (MRM) peak area ± SE (n = 3) from 5 μg extracted protein is shown for peptides mapping to: (A) B1-hordeins P06470-C1 (, right y-axis), P06470-C2 (, left y-axis), and P06470-C3 (, right y-axis); (B): B3-hordeins I6SW30-C1 (, left y-axis), I6SJ26-C1 , right y-axis), I6SJ26-C2 (, left y-axis); (C) C-hordeins Q40053-C1 (), Q40053-C3 () and Q41210-C4 (); (D) D-hordein I6TRS8-C1 (), I6TRS8-C5 () and I6TRS8-C6 (); (E) γ1-hordein TC131355-C1 (), γ1-hordein I6TMV6-C1 (); (F) γ3-hordein I6TEV2-C1 (, left y-axis), γ3 I6TEV2-C2 (, right y-axis), and γ3 I6TEV2-C3 (, right y-axis). The sequence of the proteins and peptide markers are shown in Supplementary Table S4. Where the SE is less than symbol size it is not shown. For clarity, symbols of significance are not shown but where points differ by 2 × SE they are significantly different. Detailed statistical comparisons are shown in Supplementary Figures.