Figure 1.

GSH is important in P. aeruginosa pathogenicity. (A) ΔgshB and ΔgshAΔgshB exhibited weaker swimming, swarming, and twitching motilities. The experiments were repeated at least three times with similar results. Images from one typical experiment are shown. (B) Biofilms of PAO1, ΔgshB and ΔgshAΔgshB grown in flow cells for 72 h and examined by confocal laser scanning microscopy. Experiments were performed in triplicate independently for three times. Representative images are shown. (C,D) Biofilm formation of PAO1, ΔgshB and ΔgshAΔgshB on borosilicate tube surface and quantified by the crystal violet (CV) staining methods. The results are averages based on three replicate tubes. (E) H&E stained mouse lungs 16 h post-infection by indicated P. aeruginosa strains. (F) ΔgshB and ΔgshAΔgshB are attenuated in acute pneumonia infection. Bacterial burden in CD-1 mouse lungs (n = 6) after 16 h of infection by various P. aeruginosa strains. Data represent average CFU/lung ± standard deviations. ***p < 0.001, when compared PAO1 to ΔgshB and ΔgshAΔgshB.