Table 1.
Genes with altered expression in ΔgshAΔgshB when compared to parental wild-type PAO1.
| Locus tag | Gene name | Fold change | Gene function |
|---|---|---|---|
| T3SS GENES | |||
| PA0044 | exoT | −4.45466758 | exoenzyme T |
| PA 1689 | PA1689 | 2.071591324 | Conserved hypothetical protein in T3SS |
| PA 1694 | pscQ | −2.35035367 | Translocation protein in T3SS secretion |
| PA 1695 | pscP | −2.19640736 | Translocation protein in T3SS secretion |
| PA 1701 | pcr3 | −2.363138 | Regulatory protein in T3SS |
| PA 1703 | pcrD | −2.55773203 | T3SS secretory apparatus protein PcrD |
| PA 1704 | pcrR | −3.75650633 | Transcriptional regulator protein PcrR |
| PA 1705 | pcrG | −3.18167213 | Regulator in T3SS secretion |
| PA1706 | pcrV | −3.38478764 | T3SS secretion protein PcrV |
| PA1707 | pcrH | −5.92843804 | Regulatory protein PcrH |
| PA1708 | popB | −4.78755524 | Translocator protein PopB |
| PA1709 | popD | −4.43846198 | Translocator outer membrane protein PopD precursor |
| PA1710 | exsC | −2.75707595 | ExsC, exoenzyme S synthesis protein C precursor |
| PA1711 | exsE | −4.08813812 | Regulatory protein ExsE in T3SS |
| PA1712 | exsB | −2.46600588 | Exoenzyme S synthesis protein B |
| PA1713 | exsA | −2.57563098 | Transcriptional regulator ExsA |
| PA1714 | exsD | −2.20197725 | Regulatory protein ExsD in T3SS |
| PA1715 | pscB | −2.26345008 | T3SS export apparatus protein |
| PA1716 | pscC | −2.60924525 | T3SS secretion outer membrane protein PscC precursor |
| PA1717 | pscD | −3.02363447 | T3SS export protein PscD |
| PA1718 | pscE | −2.90851002 | T3SS export protein PscE |
| PA1719 | pscF | −2.56778518 | T3SS export protein PscF |
| PA1720 | pscG | −3.62091142 | T3SS export protein PscG |
| PA1721 | pscH | −2.2775302 | T3SS export protein PscH |
| PA1722 | pscI | −2.43668146 | T3SS export protein PscI |
| PA1723 | pscJ | −2.16915564 | T3SS export protein PscJ |
| PA2191 | exoY | −3.28248108 | Adenylate cyclase ExoY |
| T6SS GENES | |||
| PA0074 | ppkA | −2.11898918 | Serine/threonine protein kinase PpkA |
| PA0077 | icmF1 | −6.31173974 | Export apparatus IcmF1 in T6SS |
| PA0078 | tssL1 | −2.15686054 | Export apparatusTssL1 in T6SS |
| PA0083 | tssB1 | −6.21885291 | Protein secretion/export apparatus in T6SS |
| PA0084 | tssC1 | −5.92072778 | Protein secretion/export apparatus in T6SS |
| PA0085 | hcp1 | −3.77657278 | Secreted Factors Hcp1 in T6SS |
| PA0086 | tagJ1 | −2.14834322 | Secretion protein in T6SS |
| PA0087 | tssE1 | −5.52513332 | Secretion protein in T6SS |
| PA0088 | tssF1 | −4.8468817 | Secretion protein in T6SS |
| PA0089 | tssG1 | −4.6167897 | Secretion protein in T6SS |
| PA0090 | clpV1 | −3.68195792 | ClpA/B–type chaperone |
| PA0091 | vgrG1 | −4.77451624 | VgrG1 in T6SS |
| PA1656 | hsiA2 | −5.30038961 | Protein secretion/export apparatus |
| PA1657 | hsiB2 | −2.06590215 | Protein secretion/export apparatus |
| PA1658 | hsiC2 | −2.42591207 | Protein secretion/export apparatus |
| PA1659 | hsiF2 | −2.82575912 | Protein secretion/export apparatus |
| PA1660 | hsiG2 | −3.8839074 | Protein secretion/export apparatus |
| PA1661 | hsiH2 | −4.41376322 | Protein secretion/export apparatus |
| PA1662 | clpV2 | −2.01011682 | Protein secretion/export apparatus |
| PA1663 | sfa2 | −3.1571465 | Protein secretion/export apparatus |
| PA1664 | orfX | −4.14296746 | Protein secretion/export apparatus |
| PA1665 | fha2 | −2.50737402 | Protein secretion/export apparatus |
| PA1666 | lip2 | −2.51739551 | Protein secretion/export apparatus |
| PA1667 | hsiJ2 | −4.52360165 | Protein secretion/export apparatus |
| PA1668 | dotU2 | −2.53779029 | Protein secretion/export apparatus |
| PA1669 | icmF2 | −2.71554212 | Protein secretion/export apparatus |
| PA1670 | stp1 | −4.28588287 | Protein secretion/export apparatus |
| PA1671 | stk1 | −5.62977334 | Protein secretion/export apparatus |
| TYPE 4 PILI BIOGENESIS GENES | |||
| PA0410 | pilI | −7.94797102 | Twitching motility protein PilI |
| PA0411 | pilJ | −10.0863067 | Twitching motility protein PilJ |
| PA0412 | pilK | −9.58293728 | Methyltransferase PilK |
| PA0413 | chpA | −10.0450818 | Component of chemotactic signal transduction system |
| PA0414 | chpB | −6.41019426 | Probable methylesterase |
| PA0415 | chpC | −3.29003495 | Probable chemotaxis protein |
| PA0416 | chpD | −4.43607137 | Probable transcriptional regulator |