Forney 2001.

Clinical features and settings	Presenting signs and symptoms: Fever over 38 °C or headache or history of fever in the previous 72 hrs Previous treatment for malaria: Patients who were currently taking antimalarial therapy or who had been treated with antimalarial drugs within the previous two weeks were excluded Clinical setting: Outpatient malaria clinics Country: Iquitos, Peru (28% of participants); Mae Sod, Thailand (72% of participants) Malaria endemicity: Seasonal Malaria endemic species:P. falciparum and P.vivax
Participants	Sample size: 3006 enrolled, 2993 had blood collected, 2988 included (2162 Thailand, 838 Peru) Age: 15 years or over (Thailand, 72% of participants); one year or over (Peru, 28% of participants) Sex: Both males and females eligible. Actual proportions of males and females in the participant population not stated. Co‐morbidities and pregnancy: No exclusion criteria based on co‐morbidities or pregnancy. No details of the frequency of these conditions in the participant population is presented. Parasite density of microscopy positive cases: Not presented
Study design	Enrollment was consecutive and prospective. One RDT was evaluated.
Target condition and reference standard(s)	Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood film Person(s) performing microscopy: Trained, certified microscopist Microscopy setting: Malaria testing station Number of high power fields examined before declaring negative: 200 Number of observer or repeats: Two independent observers Resolution of discrepancies between observers: A third senior microscopist examined both microscopist A's and microscopist B's slides, and gave the final judgement
Index and comparator tests	Commerical name of RDT: ParaSight‐F (Beckton Dickinson, Franklin Lakes, NJ, US) Parasite(s) designed to detect:P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Trained investigator and technician, with quality control checks by the principal investigator RDT setting: Malaria testing station
Follow‐up	Not applicable
Notes	Source of funding: Main funding source not stated. RDT kits provided by the manufacturer.
Table of Methodological Quality
Item	Authors' judgement	Description
Representative spectrum? All tests	Yes	Participants were a consecutive series of people attending malaria clinics with fever over 38 °C or headache or history of fever in the previous 72 hrs
Acceptable reference standard? All tests	Yes	Two independent trained microscopists viewed at least 200 high powered fields before declaring negative
Partial verification avoided? All tests	Yes	All participants who received the index test also received the reference test
Differential verification avoided? All tests	Yes	The same reference test was used regardless of the index test results
Incorporation avoided? All tests	Yes	The index test does not form part of the reference standard
Reference standard results blinded? All tests	Yes	In all cases, the results of the ParaSight‐F test were determined prior to diagnostic microscopy, with strict blinding between the rapid test results and technicians performing the microscopy
Index test results blinded? All tests	Yes	In all cases, the results of the ParaSight‐F test were determined prior to diagnostic microscopy, with strict blinding between the rapid test results and technicians performing the microscopy
Uninterpretable results reported? All tests	Yes	Tests whose results were reported as uninterpretable after the initial procedure were repeated in an attempt to resolve the discrepant event. Repeatedly uninterpretable results were reported as such and were incorporated into calculation of sensitivity and specificity as 'false' results. There were a total of seven uninterpretable results; all were negative for P. falciparum by blood slide.
Withdrawals explained? All tests	Yes	There were five withdrawals after collection of blood samples; reasons were documented in all cases.