Kyabayinze 2008.

Clinical features and settings	Presenting signs and symptoms: History of fever in the previous 24 h or axillary temperature 37.5 °C or over; no evidence of concomitant febrile illness; no danger signs or evidence of severe malaria Previous treatment for malaria: No exclusion criteria based on antimalarial use; data collected on prior antimalarial use but not presented for the study sample Clinical setting: Regional referral hospital outpatient department Country: Uganda Malaria endemicity: Hyperendemic Malaria endemic species: Mainly P. falciparum
Participants	Sample size: 357 Age: Inclusion criteria over the age of six months; 46% were under the age of five; median age 11 years (range 1 to 28 years) Sex: Both males and females eligible; 60% were female and 40% male Co‐morbidities and pregnancy: People with evidence of concomitant febrile illness were excluded from the study Parasite density of microscopy positive cases: Not presented
Study design	Enrollment was consecutive and prospective. One RDT was evaluated.
Target condition and reference standard(s)	Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood smears Person(s) performing microscopy: Hospital microscopists Microscopy setting: Hospital laboratory Number of high power fields examined before declaring negative: 100 Number of observer or repeats: Two in most cases. A third microscopist read a random 10% of slides for quality control. Resolution of discrepancies between observers: A third external microscopist, unaware of the first two results, resolved any discordant results
Index and comparator tests	Commerical name of RDTs: ICT Malaria‐Pf (ICT Diagnostics, Brookvale, NSW, Australia) Parasite(s) designed to detect:P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Storage temperature ranged from 22 °C to 29 °C, and a single spike temperature of 38 °C was recorded during transportation Person(s) performing RDT: Two independent readings by trained staff RDT setting: Hospital laboratory
Follow‐up	Not applicable
Notes	Source of funding: UK DFID through the COMDIS research programme consortium
Table of Methodological Quality
Item	Authors' judgement	Description
Representative spectrum? All tests	Yes	Participants were a consecutive series of people with fever attending outpatient clinics
Acceptable reference standard? All tests	Yes	Two microscopists working in a central laboratory viewed at least 100 high power fields before declaring a slide negative
Partial verification avoided? All tests	Yes	All participants who received the index test also received the reference test
Differential verification avoided? All tests	Yes	The same reference test was used regardless of the index test results
Incorporation avoided? All tests	Yes	The index test does not form part of the reference standard
Reference standard results blinded? All tests	Unclear	Blinding not described
Index test results blinded? All tests	Unclear	Blinding not described
Uninterpretable results reported? All tests	Yes	7 enrolled participants were excluded due to uninterpretable blood smears
Withdrawals explained? All tests	Yes	7 enrolled participants were excluded due to uninterpretable blood smears; otherwise there were no withdrawals