Sayang 2009.

Clinical features and settings	Presenting signs and symptoms: Fever or history of fever in the previous 24 h, and nurse's suspicion of malaria Previous treatment for malaria: Patients who reported taking antimalarial drugs in the previous two weeks were excluded Clinical setting: Primary health centre Country: Yoaunde, Cameroon Malaria endemicity: Reported to be high Malaria endemic species:P. falciparum
Participants	Sample size: 153 Age: Both adults and children were included; there is no indication of the proportion of adults and children in the sample Sex: Not mentioned either as an exclusion criteria or a characteristic of included participants Co‐morbidities and pregnancy: Not mentioned either as an exclusion criteria or a characteristic of included participants Parasite density of microscopy positive cases: Range 40 to 125,000 parasites per μl. Geometric mean: age 0‐2 = 2869; age 3‐5 = 6440; age 6‐10 = 1580; age over 11 = 1535
Study design	Enrollment was prospective. The sampling method was initially consecutive, from which a random sample of patients assigned to RDTs. One RDTs was evaluated.
Target condition and reference standard(s)	Target condition: Malaria parasitaemia Reference standard: Microscopy Person(s) performing microscopy: Not stated Microscopy setting: Laboratory not further described Number of high power fields examined before declaring negative: Not stated Number of observer or repeats: Not stated Resolution of discrepancies between observers: Not applicable
Index and comparator tests	Commerical name of RDT: Diaspot cassette device (Acumen Diagnostics Inc, USA) Parasite(s) designed to detect:P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Nurses trained in the use of the device RDT setting: Primary Health Centre
Follow‐up	Not applicable
Notes	Source of funding: Supported by the Service de Cooperation et d'Action Culturelle of the French Embassy in Yaounde, European Union (READ‐UP project, STREP, contract no. 018602) and the Centre de Formation et Recherche en Medicine et Sante Tropicale, Marseille, France
Table of Methodological Quality
Item	Authors' judgement	Description
Representative spectrum? All tests	Yes	Participants were all attending health centre, had fever and a nurse suspected that they might have malaria. Enrolment into the main study was consecutive, and allocation to RDT study was random.
Acceptable reference standard? All tests	Unclear	No details given of number of high power fields viewed before declaring negative, or number of observers or repeats
Partial verification avoided? All tests	Yes	All participants who received the index test also received the reference test
Differential verification avoided? All tests	Yes	The same reference test was used regardless of the index test results
Incorporation avoided? All tests	Yes	The index test does not form part of the reference standard
Reference standard results blinded? All tests	Yes	Microscopy undertaken at a different site to RDTs
Index test results blinded? All tests	Yes	RDTs undertaken before microscopy, and at a different site
Uninterpretable results reported? All tests	Unclear	The number of participants originally enrolled in the study was not clearly stated, therefore it is unclear whether there were any exclusions due to invalid test results
Withdrawals explained? All tests	Unclear	The number of participants originally enrolled in the study was not clearly stated, therefore it is unclear whether there were any withdrawals