Figure 6.

Mix multi-target VLP-based vaccine increased CD8⁺ T-cell infiltration into B16F10 tumor and enhanced the survival. (A) Density of CD8⁺ T-cells (means ± SEM) in tumor, pre-gated on TILs in the groups vaccinated with Qβ(CpGs)-VLPs, anti-CD25, GL-MTV, Mutated-MTV, and Mix-MTV. The density measured by dividing the total number of CD8⁺ T-cells in each tumor by its volume. (B) Density of CD8⁺ IFN-γ⁺ secreting cells (means ± SEM) in tumor, pre-gated on TILs in the vaccinated groups. Statistical analysis by one-way ANOVA. (C) Representative FACS plots showing the total number of CD8⁺ T-cells in each tumor in the vaccinated groups. (D) Survival of mice in the vaccinated groups, mice were euthanized when the tumor reached 1,000 mm³. The arrows indicate vaccination time. Statistical analysis by log-rank test. The groups of GL-MTV, Mutated-MTV, and Mix-MTL were combined with anti-CD25mAb in all experiments. (n = 5) mice per group, one representative of 3 similar experiments is shown. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001.