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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Neuropeptides. 2018 Nov 27;73:11–24. doi: 10.1016/j.npep.2018.11.008

Figure 6. Y2R and Y5R co-localize in CHO-K1/Y2R-mCherry/Y5R-EGFP cells.

Figure 6

A. Expression of Y2R-mCherry and Y5R-EGFP in CHO-K1 cells transfected with both constructs, detected by Western blot with anti-dsRed and anti-EGFP antibody, respectively. B. p44/42 MAPK activation in response to treatment with Y2R- and Y5R-selective agonists ([ahx(5-24)]NPY and BWX 46, respectively) administered for 5 min at a concentration of 10−7M. C. Fluorescence microscopy images of CHO-K1 cells expressing Y2R-mCherry and Y5R-EGFP proteins.