Figure 1. Nlrc3 Is Down-regulated upon TCR Stimulation, Suppressing CD4⁺ T Cell Activation.

(A) Nlrc3 expression in splenic CD4⁺ T cells and CD8⁺ T cells from WT mice after activation by anti-CD3 (5 μg/mL) and anti-CD28 (2 μg/mL) antibodies.

(B) Nlrc3 expression in splenic CD4⁺ T cells from WT mice upon activation by anti-CD3 (5 μg/mL) and anti-CD28 (2 μg/mL) antibodies under Th0, Th1, and Th17 cell conditions.

(C) CD4⁺ T cells isolated from WT and Nlrc3^−/− mice were stimulated with PMA (50 ng/mL) and ionomycin (1 μg/mL). Graphs show the percentage of CD4⁺CD25⁺ and CD4⁺CD69⁺ cells, MFI of CD25 among CD4⁺CD25⁺ cells, and MFI of CD69 among CD4⁺CD69⁺ cells after stimulation.

(D) Cells isolated from WT and Nlrc3^−/− mice were stimulated with anti-CD3 (5 μg/mL) and anti-CD28 (2 μg/mL) antibodies. Graphs show the percentage of CD4⁺CD25⁺ and CD4⁺CD69⁺ cells, MFI of CD25 among CD4⁺CD25⁺ cells, and MFI of CD69 among CD4⁺CD69⁺ cells.

Data are from one experiment representative of two or three experiments and are shown as mean ± SEM of triplicate samples. Statistical significance was determined by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001. See also Figures S1 and S2.